Ready-to-Use HPLC Applications

Simultaneous Isocratic Determination of Five Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) Using HPLC-UV

Document #030320-04. Revision 01, 03 March 2020



Acetylsalicylic acid, Ketoprofen, Naproxen, Ibuprofen, Ketorolac.

Scope of The Method

The method is intended for the isocratic determination of five nonsteroidal anti-inflammatory drugs (acetylsalicylic acid, ketoprofen, naproxen, ibuprofen, ketorolac) in various pharmaceutical and biological samples using simple isocratic 400 bar HPLC system with a conventional UV detector.

Minimum System Requirements
Solvent Delivery System: Isocratic HPLC pump with 400 bar upper back-pressure limit 
Detector: Single wavelength UV detector
Column Oven: Preferable to ensure retention time stability

Compartible with all HPLC systems: Agilent, Shimadzu, Waters, Thermo, etc.

General Information On The Method
Elution Mode: isocratic,  Detection: UV, Actual Analysis Time: 10.0 minutes. Back-pressure: typically < 300 bar at 1.5 mL/min, HPLC column manufacturer: Advanced Chromatography Technologies, Scotland >>


Fig. 1. Isocratic determination of five nonsteroidal anti-inflammatory drugs. Detection: UV 215 nm + 260 nm. 1. Acetylsalicylic acid, 2. Ketoprofen, 3. Naproxen, 4. Ibuprofen, 5. Ketorolac.


The method may require soft adjustment to provide an excellent result for a particular sample. Retention and separation selectivity tuning recommendations are given in the application.

        We can fine-tune method performance to meet your requirements upon request.

        We can develop the complete HPLC method based on this application upon request.

Table of contents

Description of The method  5
Name of The Method 5
Scope of The Method  5
Main Characteritics of The Method 5
Minimum System Requirements 5
Analyte(s) 6
Standard HPLC Conditions 6
Typical Chromatogram(s) 7
Suitable HPLC Column(s) 7
Quickstart Steps 8
Mobile Phase Preparation 8
Column Washing And Storage 8
Column Conditioning 8
Testing The Column Performance 8
Fine-Tuning Retention  9