Free Library of (U)HPLC Separations

Chromatograms & Decription

A1 Isocratic HPLC separation of ten NSAIDs – nonsteroidal anti-inflammatory drugs. Acetylsalicylic acid, Ketorolac, Piroxicam, Ketoprofen, Naproxen, Meloxicam, Nimesulide, Indometacin, Dichlofenac, Ibuprofen.

LC Mode: RP
SP: HALO 90A AQ-C18, 250x4.6, 2.7um SPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 55:45
F: 1.2 mL/min. Temp.: Ambient.
UV: 215 nm / 260 nm.

Hplc.today Isocratic HPLHplc.today Isocratic HPLC separation of nine antihypertensive drugs: diuretics, CCBs, ARBs, alpha-blockers & beta-blockers. Propranolol, Amlodipine, Doxazosin, Verapamil, Furosemide, Losartan, Indapamide, Nifedipine, Valsartan

A2 Isocratic HPLC separation of nine antihypertensive drugs: diuretics, CCBs, ARBs, alpha-blockers & beta-blockers. Propranolol, Amlodipine, Doxazosin, Verapamil, Furosemide, Losartan, Indapamide, Nifedipine, Valsartan.

LC Mode: RP/HILIC
SP: HALO 90A ES-CN, 250x4.6, 2.7um SPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 40:60
F: 1.2 mL/min. Temp.: Ambient.
UV: 240 nm / 260 nm.

A3 Isocratic HPLC separation of seven artificial sweeteners & preservatives. Saccharine, Cyclamate, Acesulfame, Caffeine, Aspartame, Sorbic acid, Benzoic acid.

LC Mode: RP/HILIC
SP: HALO 90A ES-CN, 250x4.6, 2.7um SPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 10:90
F: 2.0 mL/min. Temp.: 40C.

UV: 200 nm.

A4 Isocratic HPLC separation of antidepressant drugs. Venlafaxine, Citalopram, Duloxetin, Sertraline.

LC Mode: RP/HILIC
SP: HALO 90A ES-CN, 250x4.6, 2.7um SPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 40:60
F: 1.2 mL/min. Temp.: Ambient.
UV: 230 nm / 240 nm.

A5 Isocratic HPLC separation of alpha-blocker and beta-blocker drugs. Pindolol, Metoprolol, Prazosin, Bisoprolol, Alfuzosin, Propranolol.

LC Mode: RP/HILIC
SP: HALO 90A ES-CN, 250x4.6, 2.7um SPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 20:80
F: 1.2 mL/min. Temp.: Ambient.
UV: 225 nm / 254 nm.

A6 Isocratic HPLC separation of fluoroquinolone antibiotics. Ofloxacin, Lomefloxacin, Ciprofloxacin, Enrofloxacin, Moxifloxacin.

LC Mode: RP
SP: HALO 90A ES-CN, HALO 90A AQ-C18, 250x4.6, 2.7um SPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 20:80
F: 1.2 mL/min. Temp.: Ambient.
UV: 260 nm / 290 nm.

A7 Isocratic HPLC separation of corticosteroid drugs. Prednisolone, Prednisone, Betamethazone, Dexamethazone, Hydrocortisone acetate.

LC Mode: RP
SP: HALO 90A PFP, HALO 90A AQ-C18, 250x4.6, 2.7um SPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 30:70
F: 1.2 mL/min. Temp.: Ambient.
UV: 260 nm / 280 nm.

A8 Isocratic HPLC separation of sulfamide antibiotics. Trimethoprim, Sulfamerazine, Sulfamethazine, Sulfamethoxazole, Sulfisoxazole.

LC Mode: RP
SP: HALO 90A AQ-C18, 250x4.6, 2.7um SPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 25:75
F: 1.2 mL/min. Temp.: Ambient.
UV: 260 nm / 290 nm.

A9 Isocratic Stability-Indicating HPLC separation of Beta-Lactame Antibiotics. Cefepime, Meropenem, Cefuroxime, Cefotaxime.

LC Mode: RP
SP: HALO 90A ES-CN, 250x4.6, 2.7um SPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 12:88
F: 1.2 mL/min. Temp.: Ambient.
UV: 260 nm / 290 nm.

A10 HPLC test of RP column. Theobromine, Paracetamol, Caffeine.

LC Mode: RP
SP: HALO 160A ES-CN, 250x4.6, 2.7um SPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 5:95
F: 1.0 mL/min. Temp.: Ambient.

A10 HPLC test of HILIC column. p-Carboxymethyl Aniline, p-Aminomethyl Benzoic Acid.

LC Mode: HILIC
SP: HALO 90A HILIC, 250x4.6, 2.7um SPP
MP: AcN-(25mM NH4HCOO) 80:20
F: 1.25 mL/min. Temp.: Ambient.

Hplc.today Isocratic HPLC separation of six water soluble vitamins & their phosphates. Riboflavin, Pyridoxine, Ascorbic acid, Folic acid, Riboflavin-phosphate isomers, Pyridoxine-phosphate

E4 Isocratic HPLC separation of six water soluble vitamins & their phosphates. Riboflavin, Pyridoxine, Ascorbic acid, Folic acid, Riboflavin-phosphate isomers, Pyridoxine-phosphate.

LC Mode: SAX + HILIC
SP: Exsil Pur 120 SAX, 250x4.6, 3um FPP
MP: AcN-(50mM NH4HPO4+0.05%H3PO4) 65:35
F: 1.5 mL/min. Temp.: Ambient.
UV: 260 nm / 300 nm.

hplc.today Isocratic HPLC separation of caffeoyltartaric & caffeoylquinic acids. Chlorogenic acid, trans-Coutaric acid, Caftaric acid, Chicoric acid

E5 Isocratic HPLC separation of caffeoyltartaric & caffeoylquinic acids. Chlorogenic acid, trans-Coutaric acid, Caftaric acid, Chicoric acid.

LC Mode: SAX
SP: Exsil Pur 120 SAX, 250x4.6, 3um FPP
MP: AcN-(50mM NH4HPO4+0.05%H3PO4) 60:40
F: 1.5 mL/min. Temp.: Ambient.
UV: 270 nm / 330 nm.

Hplc.today Isocratic HPLC separation of sorbic acid, benzoic acid & ascorbic acid

E6 Isocratic HPLC separation of sorbic acid, benzoic acid & ascorbic acid.

LC Mode: SAX
SP: Exsil Pur 120 SAX, 250x4.6, 3um FPP
MP: AcN-(50mM NH4HPO4) 45:55
F: 1.5 mL/min. Temp.: Ambient.
UV: 230 nm / 260 nm.

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Hplc.today Isocratic HPLC separation of five widely used veterinary antibiotics of different classes. Metronidazole, Amoxicillin, Tiamulin, Clindamycin, Trimethoprim

H1 Isocratic HPLC separation of five widely used veterinary antibiotics of different classes in ion-exchange mode. Metronidazole, Amoxicillin, Tiamulin, Clindamycin, Trimethoprim.

LC Mode: IC (SCX)
SP: Coresep S, 150x4.6, 2.7um SPP
MP: AcN-(25mM NH4HPO4+0.2%H3PO4) 40:60
F: 1.0 mL/min. Temp.: Ambient.

Hplc.today Isocratic HPLC separation of nine preservatives. Benzyl alcohol, Dehydroacetic acid, Methyl Paraben, Ethyl Paraben, 4-Hedroxybenzoic acid, Propyl Paraben, Butyl Paraben, Sorbic acid, Benzoic acid

H2 Isocratic HPLC separation of nine preservatives. Benzyl alcohol, Dehydroacetic acid, Methyl Paraben, Ethyl Paraben, 4-Hedroxybenzoic acid, Propyl Paraben, Butyl Paraben, Sorbic acid, Benzoic acid.

LC Mode: IC (SAX) + RP
SP: Coresep SB, 150x4.6, 2.7um SPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 60:40
F: 1.0 mL/min. Temp.: Ambient.

Hplc.today Isocratic HPLC separation of six catecholamines & serotonin. DOPA, Noradrenaline, Adrenaline, Normetanephrine, Dopamine, Metanephrine, Serotonin

H3 Isocratic HPLC separation of six catecholamines & serotonin. DOPA, Norepinephrine, Epinephrine, Normetanephrine, Dopamine, Metanephrine, Serotonin.

LC Mode: IC (SCX) + RP
SP: Coresep 100, 150x4.6, 2.7um SPP
MP: AcN-(25mM NH4HPO4+0.05%H3PO4) 50:50
F: 1.0 mL/min. Temp.: Ambient.

V1 Isocratic HPLC separation of six antibiotics used for veterinaty purposes. Trimethoprim, Sulfamerazine, Oxytetracycline, Ofloxacin, Sulfamethazine, Enrofloxacin.

LC Mode: RP
SP: Ace Excel Super C18, 150x4.6, 3um FPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 15:85
F: 1.5 mL/min. Temp.: Ambient.

T1 Isocratic HPLC separation of six antibiotics used for veterinaty purposes. Trimethoprim, Sulfamerazine, Oxytetracycline, Ofloxacin, Sulfamethazine, Enrofloxacin.

LC Mode: HILIC
SP: Tracer Excel 120 APS, 250x4.6, 3um FPP
MP: AcN-(20mM NH4HCOO) 75:25
F: 1.5 mL/min. Temp.: Ambient.

E1 Isocratic HPLC separation of widely used veterinary antibiotics of different classes in ion-exchange mode. Metronidazole, Tylosin A, Tiamulin, Amoxycillin, Oxytetracycline, Lincomycin.

LC Mode: IC (SCX)
SP: Reprosil 100 SCX, 250x4.6, 3um FPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 50:50
F: 1.5 mL/min. Temp.: Ambient.

E2 Isocratic HPLC separation of six beta-lactame antibiotics of different classes in HILIC mode. Cefuroxime, Cefotaxime, Cefepime, Ampicillin, Amoxycillin.

LC Mode: HILIC
SP: Exsil Pur 120 Amino, 250x4.6, 3um FPP
MP: AcN-(20mM NH4HCOO) 80:20
F: 2.0 mL/min. Temp.: Ambient.

T2 Isocratic HPLC separation of six beta-lactame antibiotics of different classes in HILIC mode. Cefuroxime, Cefotaxime, Cefepime, Ampicillin, Amoxycillin.

LC Mode: HILIC
SP: Tracer 120 APS, 250x4.6, 3um FPP
MP: AcN-(20mM NH4HCOO) 80:20
F: 2.0 mL/min. Temp.: Ambient.

T3 Isocratic HPLC separation of fluoroquinolone antibiotics in HILIC mode. Ofloxacin, Lomefloxacin, Ciprofloxacin, Enrofloxacin, Moxifloxacin.

LC Mode: HILIC
SP: Tracer 120 APS, 250x4.6, 3um FPP
MP: AcN-(20mM NH4HCOO) 75:25
F: 1.5 mL/min. Temp.: Ambient.

T4 Isocratic HPLC separation of six antibiotics used for veterinaty purposes. Metronidazol, Trimethoprim, Sulfamerazine, Oxytetracycline, Ofloxacin, Sulfamethazine, Enrofloxacin.

LC Mode: RP
SP: Tracer Excel 120 C8, 120 ODS3.0, 250x4.6, 5um FPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 20:80
F: 1.0 mL/min. Temp.: Ambient.

T5 Isocratic HPLC separation of ten NSAIDs – nonsteroidal anti-inflammatory drugs. Acetylsalicylic acid, Ketorolac, Piroxicam, Ketoprofen, Naproxen, Meloxicam, Nimesulide, Indometacin, Dichlofenac, Ibuprofen.

LC Mode: RP
SP: Tracer Excel 120 ODS3.0, 250x4.6, 5um FPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 55:45
F: 1.0 mL/min. Temp.: Ambient.
UV: 215 nm / 260 nm.

T6 Isocratic HPLC separation of nine antihypertensive drugs: diuretics, CCBs, ARBs, alpha-blockers & beta-blockers. Propranolol, Amlodipine, Doxazosin, Verapamil, Furosemide, Losartan, Indapamide, Nifedipine, Valsartan.

LC Mode: RP
SP: Tracer Excel 120 ODS-A, 250x4.6, 5um FPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 45:55
F: 1.0 mL/min. Temp.: Ambient.
UV: 240 nm / 260 nm.

E3 Isocratic HPLC separation of ten NSAIDs – nonsteroidal anti-inflammatory drugs. Acetylsalicylic acid, Ketorolac, Piroxicam, Ketoprofen, Naproxen, Meloxicam, Nimesulide, Indometacin, Dichlofenac, Ibuprofen.

LC Mode: RP
SP: Exsil Avanti BDS Alkam, 250x4.6, 3.5um FPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 50:50
F: 1.0 mL/min. Temp.: Ambient.
UV: 215 nm / 260 nm.

Hplc.today Isocratic HPLC separation of caffeine, paracetamol & ascorbic acid

H4 Isocratic HPLC separation of Caffeine, Paracetamol, and Ascorbic acid. Caffeine, Acetaminophen, Ascorbic acid.

LC Mode: IC (SAX) + RP
SP: Coresep SB, 150x4.6, 2.7um SPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 60:40
F: 1.0 mL/min. Temp.: Ambient.

Chromatograms & Decription

A3 Isocratic HPLC separation of seven artificial sweeteners & preservatives. Saccharine, Cyclamate, Acesulfame, Caffeine, Aspartame, Sorbic acid, Benzoic acid. LC Mode: RP/HILIC SP: HALO 90A ES-CN, 250x4.6, 2.7um SPP MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 10:90 F: 2.0 mL/min. Temp.: 40C.

UV: 200 nm.

A4 Isocratic HPLC separation of antidepressant drugs. Venlafaxine, Citalopram, Duloxetin, Sertraline. LC Mode: RP/HILIC SP: HALO 90A ES-CN, 250x4.6, 2.7um SPP MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 40:60 F: 1.2 mL/min. Temp.: Ambient. UV: 230 nm / 240 nm.

A5 Isocratic HPLC separation of alpha-blocker and beta-blocker drugs. Pindolol, Metoprolol, Prazosin, Bisoprolol, Alfuzosin, Propranolol. LC Mode: RP/HILIC SP: HALO 90A ES-CN, 250x4.6, 2.7um SPP MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 20:80 F: 1.2 mL/min. Temp.: Ambient. UV: 225 nm / 254 nm.

A6 Isocratic HPLC separation of fluoroquinolone antibiotics. Ofloxacin, Lomefloxacin, Ciprofloxacin, Enrofloxacin, Moxifloxacin. LC Mode: RP SP: HALO 90A ES-CN, HALO 90A AQ-C18, 250x4.6, 2.7um SPP MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 20:80 F: 1.2 mL/min. Temp.: Ambient. UV: 260 nm / 290 nm.

A7 Isocratic HPLC separation of corticosteroid drugs. Prednisolone, Prednisone, Betamethazone, Dexamethazone, Hydrocortisone acetate. LC Mode: RP SP: HALO 90A PFP, HALO 90A AQ-C18, 250x4.6, 2.7um SPP MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 30:70 F: 1.2 mL/min. Temp.: Ambient. UV: 260 nm / 280 nm.

A8 Isocratic HPLC separation of sulfamide antibiotics. Trimethoprim, Sulfamerazine, Sulfamethazine, Sulfamethoxazole, Sulfisoxazole. LC Mode: RP SP: HALO 90A AQ-C18, 250x4.6, 2.7um SPP MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 25:75 F: 1.2 mL/min. Temp.: Ambient. UV: 260 nm / 290 nm.

A9 Isocratic Stability-Indicating HPLC separation of Beta-Lactame Antibiotics. Cefepime, Meropenem, Cefuroxime, Cefotaxime. LC Mode: RP SP: HALO 90A ES-CN, 250x4.6, 2.7um SPP MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 12:88 F: 1.2 mL/min. Temp.: Ambient. UV: 260 nm / 290 nm.

A10 HPLC test of RP column. Theobromine, Paracetamol, Caffeine. LC Mode: RP SP: HALO 160A ES-CN, 250x4.6, 2.7um SPP MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 5:95 F: 1.0 mL/min. Temp.: Ambient.

A10 HPLC test of HILIC column. p-Carboxymethyl Aniline, p-Aminomethyl Benzoic Acid. LC Mode: HILIC SP: HALO 90A HILIC, 250x4.6, 2.7um SPP MP: AcN-(25mM NH4HCOO) 80:20 F: 1.25 mL/min. Temp.: Ambient.

E5 Isocratic HPLC separation of caffeoyltartaric & caffeoylquinic acids. Chlorogenic acid, trans-Coutaric acid, Caftaric acid, Chicoric acid. LC Mode: SAX SP: Exsil Pur 120 SAX, 250x4.6, 3um FPP MP: AcN-(50mM NH4HPO4+0.05%H3PO4) 60:40 F: 1.5 mL/min. Temp.: Ambient. UV: 270 nm / 330 nm.

E6 Isocratic HPLC separation of sorbic acid, benzoic acid & ascorbic acid. LC Mode: SAX SP: Exsil Pur 120 SAX, 250x4.6, 3um FPP MP: AcN-(50mM NH4HPO4) 45:55 F: 1.5 mL/min. Temp.: Ambient. UV: 230 nm / 260 nm.