Chromatograms & Decription
A1 Isocratic HPLC separation of ten NSAIDs – nonsteroidal anti-inflammatory drugs. Acetylsalicylic acid, Ketorolac, Piroxicam, Ketoprofen, Naproxen, Meloxicam, Nimesulide, Indometacin, Dichlofenac, Ibuprofen.
LC Mode: RP
SP: HALO 90A AQ-C18, 250x4.6, 2.7um SPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 55:45
F: 1.2 mL/min. Temp.: Ambient.
UV: 215 nm / 260 nm.
A2 Isocratic HPLC separation of nine antihypertensive drugs: diuretics, CCBs, ARBs, alpha-blockers & beta-blockers. Propranolol, Amlodipine, Doxazosin, Verapamil, Furosemide, Losartan, Indapamide, Nifedipine, Valsartan.
LC Mode: RP/HILIC
SP: HALO 90A ES-CN, 250x4.6, 2.7um SPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 40:60
F: 1.2 mL/min. Temp.: Ambient.
UV: 240 nm / 260 nm.
A3 Isocratic HPLC separation of seven artificial sweeteners & preservatives. Saccharine, Cyclamate, Acesulfame, Caffeine, Aspartame, Sorbic acid, Benzoic acid.
LC Mode: RP/HILIC
SP: HALO 90A ES-CN, 250x4.6, 2.7um SPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 10:90
F: 2.0 mL/min. Temp.: 40C.
UV: 200 nm.
A4 Isocratic HPLC separation of antidepressant drugs. Venlafaxine, Citalopram, Duloxetin, Sertraline.
LC Mode: RP/HILIC
SP: HALO 90A ES-CN, 250x4.6, 2.7um SPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 40:60
F: 1.2 mL/min. Temp.: Ambient.
UV: 230 nm / 240 nm.
A5 Isocratic HPLC separation of alpha-blocker and beta-blocker drugs. Pindolol, Metoprolol, Prazosin, Bisoprolol, Alfuzosin, Propranolol.
LC Mode: RP/HILIC
SP: HALO 90A ES-CN, 250x4.6, 2.7um SPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 20:80
F: 1.2 mL/min. Temp.: Ambient.
UV: 225 nm / 254 nm.
A6 Isocratic HPLC separation of fluoroquinolone antibiotics. Ofloxacin, Lomefloxacin, Ciprofloxacin, Enrofloxacin, Moxifloxacin.
LC Mode: RP
SP: HALO 90A ES-CN, HALO 90A AQ-C18, 250x4.6, 2.7um SPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 20:80
F: 1.2 mL/min. Temp.: Ambient.
UV: 260 nm / 290 nm.
A7 Isocratic HPLC separation of corticosteroid drugs. Prednisolone, Prednisone, Betamethazone, Dexamethazone, Hydrocortisone acetate.
LC Mode: RP
SP: HALO 90A PFP, HALO 90A AQ-C18, 250x4.6, 2.7um SPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 30:70
F: 1.2 mL/min. Temp.: Ambient.
UV: 260 nm / 280 nm.
A8 Isocratic HPLC separation of sulfamide antibiotics. Trimethoprim, Sulfamerazine, Sulfamethazine, Sulfamethoxazole, Sulfisoxazole.
LC Mode: RP
SP: HALO 90A AQ-C18, 250x4.6, 2.7um SPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 25:75
F: 1.2 mL/min. Temp.: Ambient.
UV: 260 nm / 290 nm.
A9 Isocratic Stability-Indicating HPLC separation of Beta-Lactame Antibiotics. Cefepime, Meropenem, Cefuroxime, Cefotaxime.
LC Mode: RP
SP: HALO 90A ES-CN, 250x4.6, 2.7um SPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 12:88
F: 1.2 mL/min. Temp.: Ambient.
UV: 260 nm / 290 nm.
A10 HPLC test of RP column. Theobromine, Paracetamol, Caffeine.
LC Mode: RP
SP: HALO 160A ES-CN, 250x4.6, 2.7um SPP
MP: AcN-(50mM NH4HPO4+0.1%H3PO4) 5:95
F: 1.0 mL/min. Temp.: Ambient.
A10 HPLC test of HILIC column. p-Carboxymethyl Aniline, p-Aminomethyl Benzoic Acid.
LC Mode: HILIC
SP: HALO 90A HILIC, 250x4.6, 2.7um SPP
MP: AcN-(25mM NH4HCOO) 80:20
F: 1.25 mL/min. Temp.: Ambient.
E4 Isocratic HPLC separation of six water soluble vitamins & their phosphates. Riboflavin, Pyridoxine, Ascorbic acid, Folic acid, Riboflavin-phosphate isomers, Pyridoxine-phosphate.
LC Mode: SAX + HILIC
SP: Exsil Pur 120 SAX, 250x4.6, 3um FPP
MP: AcN-(50mM NH4HPO4+0.05%H3PO4) 65:35
F: 1.5 mL/min. Temp.: Ambient.
UV: 260 nm / 300 nm.
E5 Isocratic HPLC separation of caffeoyltartaric & caffeoylquinic acids. Chlorogenic acid, trans-Coutaric acid, Caftaric acid, Chicoric acid.
LC Mode: SAX
SP: Exsil Pur 120 SAX, 250x4.6, 3um FPP
MP: AcN-(50mM NH4HPO4+0.05%H3PO4) 60:40
F: 1.5 mL/min. Temp.: Ambient.
UV: 270 nm / 330 nm.
E6 Isocratic HPLC separation of sorbic acid, benzoic acid & ascorbic acid.
LC Mode: SAX
SP: Exsil Pur 120 SAX, 250x4.6, 3um FPP
MP: AcN-(50mM NH4HPO4) 45:55
F: 1.5 mL/min. Temp.: Ambient.
UV: 230 nm / 260 nm.
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- Cosmosil (Nacalai Tesque, Japan).
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