HPLC Method Development & Research
Our primary objective is providing our customers with the state-of-the-art, high-performance and cost-effective analytical and preparative HPLC methods.
We believe that truly modern HPLC methods should meet a number of stringent requirements and possess a number of characteristic features, which set them apart from outdated, cost-prohibitive, and non-robust ones.
Among such distinguishing features the following ones should be mentioned:
- focus on method's specificity;
- high plate count & peak symmetry;
- isocratic mode for routine methods;
- no mobile phase additives;
- minimum sample preparation.
Specific HILIC & IC separations that require minimum sample preparation should be the norm for pharmaceutical and food analysis. In turn, mixed-mode separations (RP/HILIC, RP/CT, RP/IC) should become the norm for RP chromatography.
The modern stationary phase should be as chemically inert as possible providing high peak symmetry for basic drugs and various chelating compounds. For 400-600 bar HPLC systems, the plate count should be preferably within the range 5'000-25'000 in case of IC separations, 8'000-40'000 in case of RP and HILIC separations, and 15'000-80'000 in case of NP separations.
Specificity, robustness, and cost-effectiveness - these three basic principles are at the core of our HPLC methods. A good HPLC method should provide the necessary accuracy, but at the same time should also be fast, inexpensive, and easy to validate and to transfer. And, certainly, it should work smoothly over the long term.
We have accumulated vast experience and expertise in solving industry-specific analytical tasks that emerge in healthcare, biotechnology, agricultural, food, chemical, oil and energy industries.
Improvement of an HPLC method involves the adjustment of separation and detection conditions in order to enhance the most essential method's characteristics without switching to a different HPLC mode, or to a different packing type.
In line with this approach, any validation characteristic such as robustness, specificity, LOD/LOQ, or any commercial characteristic such as throughput, cost-effectiveness, and simplicity can be enhanced.
Chiral HPLC methods stand head and shoulders above all other HPLC methods in terms of the amount of experience and expertise required for their development.
Chromatographic behavior of optical isomers cannot be easily predicted since it follows the rules, which are quite different from those applicable to non-chiral compounds. Fortunately, I.B.S. has required experience and capacity to develop the most sophisticated chiral HPLC methods.
I.B.S. is specialized in development of PK/PD HPLC assays with use of pre-column chemical derivatization and conventional HPLC-FLD or HPLC-UV equipment. Such approach is an ideal choice for biotech start-up companies since their primary requirements to a PK/PD assay are cost-effectiveness and robustness, along with necessary LOQ and specificity.
Isolation and identification of biologically active compounds can be a serious challenge which may have much more in common with scientific research work than with routine analytical task.
Bioactive compounds may belong to different chemical classes; they may possess different molecular weight, spectral and adsorption properties; they may occur in higher or trace concentrations. There can be several active constituents in a sample, or even several different sub-classes of compounds that demonstrate bioactivity.
HPLC combined with modern spectrometric techniques is the most suitable method for discovery and isolation of such bioactive compounds.