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Simultaneous Determination of Added Methionine, Threonine, And Lysine In Premixes Using Isocratic HPLC-RID
Document #031120-11. Revision 01, 11 March 2020
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Methionine, Threonine, Lysine.
Scope of The Method
The method is intended for the isocratic determination of added methionine, threonine, and lysine in premixes using simple isocratic 400 bar HPLC system with a conventional RID detector.
Minimum System Requirements
Solvent Delivery System: Isocratic HPLC pump with 400 bar upper back-pressure limit
Detector: RID detector
Column Oven: Preferable to ensure retention time stability
Compartible with all HPLC systems: Agilent, Shimadzu, Waters, Thermo, etc.
General Information On The Method
Elution Mode: isocratic, Detection: UV, Actual Analysis Time: 12.0 minutes, Back-pressure: typically < 200 bar, HPLC column manufacturer: Nouryon, Sweden >>
Fig. 1. Isocratic determination of added methionine, threonine, and lysine in the premix sample #1. Detection: RID.
1. Methioine, 2. Threonine, 3. Lysine.
The method may require soft adjustment to provide an excellent result for a particular sample. Retention and separation selectivity tuning recommendations are given in the application.
We can fine-tune method performance to meet your requirements upon request.
We can develop the complete HPLC method based on this application upon request.
Table of contents
Description of The method 5
Name of The Method 5
Scope of The Method 5
Main Characteritics of The Method 5
Minimum System Requirements 5
Standard HPLC Conditions 6
Typical Chromatogram(s) 7
Suitable HPLC Column(s) 7
Quickstart Steps 8
Mobile Phase Preparation 8
Column Washing And Storage 8
Column Conditioning 8
Testing The Column Performance 8
Fine-Tuning Retention And Selectivity 9