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    Ready-to-Use HPLC Applications

Simultaneous Determination of Added Methionine, Threonine, And Lysine In Premixes Using Isocratic HPLC-RID

Document #031120-11. Revision 01, 11 March 2020



Methionine, Threonine, Lysine.

Scope of The Method

The method is intended for the isocratic determination of added methionine, threonine, and lysine in premixes using simple isocratic 400 bar HPLC system with a conventional RID detector.

Minimum System Requirements
Solvent Delivery System: Isocratic HPLC pump with 400 bar upper back-pressure limit 
Detector: RID detector
Column Oven: Preferable to ensure retention time stability

Compartible with all HPLC systems: Agilent, Shimadzu, Waters, Thermo, etc.

General Information On The Method
Elution Mode: isocratic, Detection: UV, Actual Analysis Time: 12.0 minutes, Back-pressure: typically < 200 bar, HPLC column manufacturer: Nouryon, Sweden >>


Methionine, Threonine, Lysine, premixes, HPLC, Agilent, Shimadzu, Waters, Thermo Scientific

Fig. 1. Isocratic determination of added methionine, threonine, and lysine in the premix sample #1. Detection: RID.
1. Methioine,  2. Threonine, 3. Lysine.

Methionine, Threonine, Lysine


The method may require soft adjustment to provide an excellent result for a particular sample. Retention and separation selectivity tuning recommendations are given in the application.

        We can fine-tune method performance to meet your requirements upon request.

        We can develop the complete HPLC method based on this application upon request.

Table of contents

Description of The method  5
Name of The Method 5
Scope of The Method  5
Main Characteritics of The Method 5
Minimum System Requirements 5
Analyte(s) 6
Standard HPLC Conditions 6
Typical Chromatogram(s) 7
Suitable HPLC Column(s) 7
Quickstart Steps 8
Mobile Phase Preparation 8
Column Washing And Storage 8
Column Conditioning 8
Testing The Column Performance 8
Fine-Tuning Retention And Selectivity 9


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