Integrated BioSeparation Solutions
Outsourcing HPLC Method Development & Validation Services. In-House HPLC Courses
Specificity, robustness, and cost-effectiveness - these three basic principles are at the core of our HPLC methods. A good HPLC method should provide the necessary accuracy, but at the same time should also be fast, inexpensive, easy to validate and to transfer. And, certainly, it should work smoothly over the long term.
We have accumulated vast experience and expertise in solving industry-specific analytical tasks that emerge in healthcare, biotechnology, agricultural, food, chemical, oil and energy industries.
Improvement of an HPLC method involves the adjustment of separation and detection conditions in order to enhance the most essential method's characteristics without switching to a different HPLC mode, or to a different packing type.
In line with this approach, any validation characteristic such as robustness, specificity, LOD/LOQ, or any commercial characteristic such as throughput, cost-effectiveness, and simplicity can be enhanced.
Discovery of Bioactive Compounds
Isolation and identification of biologically active compounds can be a serious challenge which may have much more in common with scientific research work than with routine analytical task.
Bioactive compounds may belong to different chemical classes; they may possess different molecular weight, spectral and adsorption properties; they may occur in higher or trace concentrations. There can be several active constituents in a sample, or even several different sub-classes of compounds that demonstrate bioactivity.
HPLC combined with modern spectrometric techniques is the most suitable method for discovery and isolation of such bioactive compounds.
HPLC Method Development & Research
The aim of this project is to provide our customers with reasonably priced (25-100$), state-of-the-art HPLC approaches that can be used to develop the most cost-effective commercial HPLC methods easily and quickly.
Given HPLC solutions are optimized to be used with optical detectors (UV/Vis, RID, FLD).
We can:
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optimize them to be used with evaporative (ELSD, MS) detectors,
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fine-tune method performance to meet your requirements, or
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develop the complete HPLC method based on the given application upon request.
Ready-to-Use HPLC Applications
This unique 3-days HPLC course gives all necessary tools for quick and easy development of state-of-the-art, high performance, high-throughput & cost-efficient HPLC methods. It is the in-house course, and the price is fixed (10'000$), i.e. it does not depend on the quantity of participants in the group.
From this HPLC method development course you will learn:
- how to predict behavior of a target molecule in any single or mixed HPLC mode using its structural formula;
- how to select optimal stationary phase for a given HPLC separation in RP, HILIC, IC, NP or mixed HPLC mode;
- what are the standard mobile phase compositions, and how to use mobile phase adjustments for fine-tuning retention and separation selectivity in PR, NP, HILIC and IC modes;
- how to choose optimal flow rate, column length and particle size for the developed HPLC separation;
- how to organize HPLC method development process in order to get an outstanding HPLC method just in few days.
- how to take full control of your HPLC column’s chemistry, and understand it better than the column’s producer;
- why a HILIC separation can be hard to reproduce, and how to develop a really robust HILIC separation;
- how to control selectivity and retention in ion-pairing RP mode.
- how to avoid sample preparation and get rid of matrix components with use of HILIC, IC and NP modes,
- how to convert an ion-pairing separation into a standard IC, HILIC, or mixed RP one,
- how to convert a gradient RP separation into an isocratic one in mixed RP mode,
- how to develop an MS-compatible HILIC or IC separation.