HPLC Method Development & Research  

The aim of this project is to provide our customers with reasonably priced (25-100$), state-of-the-art HPLC approaches that can be used to develop the most cost-effective commercial HPLC methods easily and quickly.

  Given HPLC solutions are optimized to be used with optical detectors (UV/Vis, RID, FLD).

   We can:

  • optimize them to be used with evaporative (ELSD, MS) detectors,

  • fine-tune method performance to meet your requirements, or

  • develop the complete HPLC method based on the given application upon request.

  Ready-to-Use HPLC Applications   

This unique 3-days HPLC course gives all necessary tools for quick and easy development of state-of-the-art, high performance, high-throughput & cost-efficient HPLC methods. It is the in-house course, and the price is fixed (10'000$), i.e. it does not depend on the quantity of participants in the group.

    From this HPLC method development course you will learn:

- how to predict behavior of a target molecule in any single or mixed HPLC mode using its structural formula;
- how to select optimal stationary phase for a given HPLC separation in RP, HILIC, IC, NP or mixed HPLC mode;
- what are the standard mobile phase compositions, and how to use mobile phase adjustments for fine-tuning retention and separation selectivity in PR, NP, HILIC and IC modes;
- how to choose optimal flow rate, column length and particle size for the developed HPLC separation;
- how to organize HPLC method development process in order to get an outstanding HPLC method just in few days.
- how to take full control of your HPLC column’s chemistry, and understand it better than the column’s producer; 
-  why a HILIC separation can be hard to reproduce, and how to develop a really robust HILIC separation;
- how to control selectivity and retention in ion-pairing RP mode.
- how to avoid sample preparation and get rid of matrix components with use of HILIC, IC and NP modes,
- how to convert an ion-pairing separation into a standard IC, HILIC, or mixed RP one,
- how to convert a gradient RP separation into an isocratic one in mixed RP mode,
- how to develop an MS-compatible HILIC or IC separation.