Specificity, robustness, and cost-effectiveness. These three basic principles are at the core of our HPLC methods.
A good HPLC method should provide the necessary accuracy, but at the same time also should also be fast, inexpensive and easy to apply, to validate and to transfer. And, certainly, it should work smoothly over the long term.
Our team achieves all these objectives by using the innovative approach that exploits state-of-the-art three-step method development workflow.. >>
Improvement of an HPLC method involves the adjustment of separation and detection conditions in order to enhance the most essential method's characteristics without switching to a different HPLC mode, or to a different packing type.
In line with this approach, any validation characteristic such as robustness, specificity, LOD/LOQ, or any commercial characteristic such as throughput, cost-effectiveness, and simplicity can be enhanced.
The main directions of an HPLC method improvement are the following >>
HPLC Solutions
I.B.S. has accumulated vast experience and expertise in solving industry-specific analytical tasks that emerge in healthcare, biotechnology, agricultural, food, chemical, oil and energy industries. >>
Company Profile
IBS (TM) specialty HPLC columns are supplied with one or several tailor-made HPLC applications aimed at solving industry-specific analytical tasks that emerge in healthcare, biotechnology, agricultural, food, chemical, oil and energy industries.
IBS (TM) applications can be customized as appropriate. In case separation obtained on an IBS (TM) column is good in general, but not perfect down to the
last detail, I.B.S. specialists can change and fine-tune the original separation conditions to suit best for a specific analytical task. Therefore, customized HPLC columns are supplied with fully customized HPLC applications developed by I.B.S. specialists.
The key feature of the entire range of our specialty HPLC solutions is using isocratic elution mode. Every IBS (TM) application exploits benefits of this approach, namely unsurpassed robustness and the highest possible separation specificity, in order to present the best specialty HPLC solution available on the market.>>
Isolation and identification of biologically active compounds can be a serious challenge which may have much more in common with scientific research work than with routine analytical task.
Since ‘discovery’ implies having very little initial information about a given specimen and its bioactive constituents, such experiments cannot be straightforward.
Bioactive compounds may belong to different chemical classes; they may possess different molecular weight, spectral and adsorption properties; they may occur in higher or trace concentrations. There can be several active constituents in a sample, or even several different sub-classes of compounds that demonstrate bioactivity. >>
Broader vision of possible pathways for solving a chromatographic separation task increases the chance to succeed in developing an appropriate HPLC method. It also helps to avoid the risk of failure, since the roots of all problems with method development ultimately lie in thinking within narrow boundaries of the only conventional approach.
The course focuses on the state-of-the-art method development workflow, which is applicable to any single or mixed HPLC mode. It aims at organizing method development process in the correct manner and thus allows performing easy and rapid method development and validation studies. >>