Specialty and Customized HPLC Columns

         IBS (TM) specialty HPLC columns are supplied with one or several tailor-made HPLC applications aimed at solving industry-specific analytical tasks that emerge in healthcare, biotechnology, agricultural, food, chemical, oil and energy industries.

 

        The key feature of the entire range of our specialty HPLC solutions is using isocratic elution mode. Every IBS (TM) application exploits benefits of this approach, namely unsurpassed robustness and the highest possible separation specificity, in order to present the best speciality HPLC solution available on the market.

 

         Every original IBS (TM) separation provided can be realized using standard 400 bar HPLC pump. Application of higher flow rates makes it possible to achieve shorter run times, although it requires the use of UHPLC equipment.

 

         IBS (TM) applications can be customized as appropriate. In case separation obtained on IBS (TM) column is good in general, but not perfect down to the last detail, our specialists can change and fine-tune the original separation conditions to suit best for a specific analytical task. Therefore, customized HPLC columns are supplied with fully customized HPLC applications developed by I.B.S. specialists.

 

         With IBS (TM) specialty and customized HPLC columns you can be sure you own the most cost-effective, straightforward and reliable HPLC solutions ever possible.

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Figure 1. Reversed-phase HPLC separation of a typical cold and flu medication components using 250x4.6 5um IBSpharm CM-1 specialty HPLC column.

  IBS (TM) Specialty and Customized Columns 
 
 
  IBSpharm Specialty Columns List 
 

Stationary phases for HPLC analysis of food, beverages and dietary supplements:

 

PS-1: Preservatives and sweeteners. >>

BV-1: Water-soluble vitamins. >>

NA-1: Nicotinamide (niacin) and nicotinic acid. >>

  IBSnutri Specialty Columns List 
 

IBSnutri PS-1: Preservatives and artificial sweeteners

Scope.    The stationary phase is designed for the determination of two preservatives (benzoic and sorbic acids), four artificial sweeteners (aspartame, acefulfam, saccharin, cyclamate) and caffeine in juices and beverages using isocratic elution mode.

HPLC analyssi of aspartame, acesulfame, saccharin, cyclamate, benzoic acid, sorbic acid and caffeine IBSnutri PS-1 HPLC column IBS

Figure 1. Group HPLC determination of aspartame, acesulfame, saccharin, cyclamate, benzoic acid, sorbic acid and caffeine.

SP: 250x4.6 IBSnutri PS-1

MP: Acetonitrile (15mM NH4H2PO4) 7:93

Flow rate: 0.8 ml/min

Column temperature: 45 °C

Benefits

1. The possibility to determine seven target compounds simultaniously under isocratic elution conditions.

2. The approach requires the conventional UV detector only (there is no need to purchase an expensive RI detector).

3. Main UV-absorbing matrix components (first of all, organic acids) are well separated from target compounds under conditions used in the application.

4. The determination of benzoic and sorbic acids can be carried out using a mobile phase that is free from organic solvents. Thus, this solution is optimal for food laboratories.

HPLC analysis of benzoic and sorbic acids in juices and beverages using the 100% aqueous mobile phase IBSnutri PS-1 HPLC column IBS

Figure 2. Group HPLC determination of benzoic and sorbic acids in juices and beverages using the 100% aqueous mobile phase. a) Chromatogram of standards, b) Chromatogram of an apple juice spiked with benzoic and sorbic acids.  

SP: 250x4.6 IBSnutri PS-1

MP: 10 mM NH4H2PO4

Flow rate: 0.8 ml/min

Column temperature: 45 °C

Comments

 

1. To determine benzoic and sorbic acids in matrices that contain fats and surfactants (for example, cosmetics, fat-containing food, etc.), we reccomend to use another stationary phase, namely IBSnutri PR-1.

2. In case preservative is ascorbic acid, it can be determined using IBSnutri PR-2 in aqueous matrices, or using IBSnutri PR-1 in fat-containing matrices.

Inquiry letter   Please, use the form below to send the inquiry letter. We will be glad to receive it, and we undertake to reply no later than 36 hours. Information on the price and stock availability will be attached to our answer. 
You can also send your inquire letter directly to our e-mail sales@hplc.today. Thank you! 

  IBSnutri PS-1  
 

IBSnutri BV-1.1: Water-soluble vitamins. Type 1.1

IBSnutri BV-1.2: Water-soluble vitamins. Type 1.2

Scope:    The stationary phase is designed for the determination of six water-soluble vitamins: ascorbic acid (vitamin C), thiamine (vitamin B1), riboflavin (vitamin B2), nicotinamide (vitamin B3), pyridoxine (vitamin B6), folic acid (vitamin B9 ) and routine (vitamin PP) in medicines, dietary supplements, energy drinks, premixes and other objects using isocratic elution mode.

HPLC analysis of water-soluble vitamins: ascorbic acid (vitamin C), thiamine (vitamin B1), riboflavin (vitamin B2), nicotinamide (vitamin B3), pyridoxine (vitamin B6), folic acid (vitamin B9) and rutin (vitamin PP) IBSnutri BV-1.1 HPLC column IBS

Figure 1. Group HPLC determination of water-soluble vitamins: ascorbic acid (vitamin C), thiamine (vitamin B1), riboflavin (vitamin B2), nicotinamide (vitamin B3), pyridoxine (vitamin B6), folic acid (vitamin B9) and rutin (vitamin PP).

SP: 250x4.6 IBSnutri BV-1.1

MP: Acetonitrile (20 mM NH4H2PO4 + 0.05% H3PO4) 80:20

Flow rate: 2.0 ml/min

Column temperature: 25 °C

Benefits

1. The possibility to determine seven target compounds simultaniously under isocratic elution conditions.

2. The mobile phase based on ammonium dihydrogen phosphate buffer solution is compatible both with UV and RI detection. To apply mass-spectrometric detection, ammonium dihydrogen phosphate should be replaced by ammonium formate, and phosphoric acid should be replaced by formic acid while maintaining the same salt and acid concentration.

3. The separation of water-soluble vitamins is characterized by very high efficiency and excellent peak symmetry; at the same time, the analysis time is less than 20 minutes, and the backpressure is less than 100 bar (10 MPa, 1500psi).

HPLC analysis of water-soluble vitamins: ascorbic acid (vitamin C), thiamine (vitamin B1), riboflavin (vitamin B2), nicotinamide (vitamin B3), pyridoxine (vitamin B6), folic acid (vitamin B9) and rutin (vitamin PP) IBSnutri BV-1 HPLC column IBS

Figure 2. Group HPLC determination of water-soluble vitamins: ascorbic acid (vitamin C), thiamine (vitamin B1), riboflavin (vitamin B2), nicotinamide (vitamin B3), pyridoxine (vitamin B6), folic acid (vitamin B9) and rutin (vitamin PP).

SP: 250x4.6 IBSnutri BV-1.2

MP: Acetonitrile (20 mM NH4H2PO4 + 0.05% H3PO4) 80:20

Flow rate: 2.0 ml/min

Column temperature: 25 °C

Benefits

1. The possibility to determine seven target compounds simultaniously under isocratic elution conditions.

2. The mobile phase based on ammonium dihydrogen phosphate buffer solution is compatible both with UV and RI detection. To apply mass-spectrometric detection, ammonium dihydrogen phosphate should be replaced by ammonium formate, and phosphoric acid should be replaced by formic acid while maintaining the same salt and acid concentration.

3. The separation of water-soluble vitamins is characterized by very high efficiency and excellent peak symmetry; at the same time, the analysis time is less than 20 minutes, and the backpressure is less than 100 bar (10 MPa, 1500psi).

Comments

1. Determination of nicotinamide (niacin) and nicotinic acid with post-column derivatization in accordance with EN 15652-2015 should be carried out using IBSnutri NA-1 specialty stationary phase.

Inquiry letter   Please, use the form below to send the inquiry letter. We will be glad to receive it, and we undertake to reply no later than 36 hours. Information on the price and stock availability will be attached to our answer. 
You can also send your inquire letter directly to our e-mail sales@hplc.today. Thank you! 

  IBSnutri BV-1  
 
  IBSnutri NA-1  

IBSnutri NA-1: Nicotinamide (niacin) and nicotinic acid

Scope:   The stationary phase is designed for the trace-level determination of nicotinamide (niacin) and nicotinic acid with post-column derivatization in accordance with EN 15652-2015 using isocratic elution mode.

HPLC analysis of nicotinamide (niacin) and nicotinic acid IBSnutri NA-1 HPLC column IBS  ​

Figure 1. Group HPLC determination of nicotinamide (niacin) and nicotinic acid.

SP: 250x4.0 IBSnutri NA-1

MP: Acetonitrile-(50mM NH4H2PO4)-H3PO4 50:50:0.25

Flow rate: 1.5 ml/min

Column temperature: 25 °C

Benefits

1. The separation of nicotinamide and nicotinic acid is characterized by very high efficiency and excellent peak symmetry; at the same time, the analysis time is less than 10 minutes.

Comments

1. To determine nicotinamide (niacin) and nicotinic acid with post-column derivatization in accordance with EN 15652-2015, post-column derivatization system and fluorometric detector are required.

Inquiry letter   Please, use the form below to send the inquiry letter. We will be glad to receive it, and we undertake to reply no later than 36 hours. Information on the price and stock availability will be attached to our answer. 
You can also send your inquire letter directly to our e-mail sales@hplc.today. Thank you! 

 
  IBSpharm AH-1   

IBSpharm AH-1: Antihypertensive drugs

 

Scope:   The stationary phase is designed for the determination of antihypertensive drugs in antihypertensive combinations: beta adrenoblockers (such as bisoprolol) and alpha-adrenobrokators (such as doxazosin), calcium channel blockers (such as amlodipine, nifedipine) and diuretics (furosemide, hydrochlorothiazide) using isocratic elution mode.

HPLC analysis of antihypertensive drugs (bisoprolol, doxazosin, amlodipine, nifedipine, furosemide, and hydrochlorothiazide) IBSpharm AH-1 HPLC column IBS

Figure 1. Group HPLC determination of bisoprolol, doxazosin, amlodipine, nifedipine, furosemide, and hydrochlorothiazide.

SP: 250x4.6 IBSpharm AH-1

MP: Acetonitrile-(50 mM NH4H2PO4 + 0.1% H3PO4) 35:65

Flow rate: 1.0 ml/min

Column temperature: 25 °C

Inquiry letter   Please, use the form below to send the inquiry letter. We will be glad to receive it, and we undertake to reply no later than 36 hours. Information on the price and stock availability will be attached to our answer. 
You can also send your inquire letter directly to our e-mail sales@hplc.today. Thank you! 

 
  IBSpharm MA-1  

IBSpharm MA-1: Pharmaceutical combinations of bronchodilator, antitussive and antispasmodic drugs

 

Scope:   The stationary phase is designed for the highly selective determination of different drugs in cough and cold medicines: bronchodilator drugs (such as salmeterol, pseudoephedrine), antitussive drugs (such as dextromethorphan, codeine), spasmolytic drugs (such as drotaverin) and diphenhydramine using isocratic elution mode.

HPLC analysis bronchodilator, antitussive and antispasmodic drugs (salmeterol, pseudoephedrine, dextromethorphan, codeine, drotaverin and diphenhydramine) IBSpharm MA-1 HPLC column IBS

Figure 1. Group HPLC determination of salmeterol, pseudoephedrine, dextromethorphan, codeine, drotaverin and diphenhydramine.

SP: 250x4 IBSpharm MA-1

MP: Acetonitrile-(70 mM NH4H2PO4 + 0.2% H3PO4) 50:50

Flow rate: 1.0 ml/min

Column temperature: 25 °C

Example 1

Application of IBSpharm MA-1 for the simultanious determination of codeine and phenyltoloxamine.

HPLC analysis of codeine and phenyltoloxamine IBSpharm MA-1 HPLC column IBS

SP: 250x4 IBSpharm MA-1

MP: Acetonitrile-Methanol-(70 mM NH4H2PO4 + 0.2% H3PO4) 35:15:50

Flow rate: 1.0 ml/min

Column temperature: 25 °C

HPLC analysis phenylephrine and pseudoephedrine IBSpharm MA-1 HPLC column IBS

Comments

1. For the separation of structurally similar compounds, it may be advantageous to use mobile phases (MP) containing a certain proportion of methanol. For example, the use of the methanol-containing MP provides the separation of phenylephrine from pseudoephedrine with use of IBSpharm MA-1.

Inquiry letter   Please, use the form below to send the inquiry letter. We will be glad to receive it, and we undertake to reply no later than 36 hours. Information on the price and stock availability will be attached to our answer. 
You can also send your inquire letter directly to our e-mail sales@hplc.today. Thank you! 

 
  IBSpharm CM-1   

IBSpharm CM-1: Cold medicines containing acetamiphen (paracetamol), caffeine, guaifenesin and phenobarbital

  

Scope:   The stationary phase is designed for the analysis of cold medicines containing acetamiphen (paracetamol), caffeine, guaifenesin and phenobarbital using isocratic elution mode.

HPLC analysis of acetaminophen (paracetamol), caffeine, guaifenesin, phenobarbital, metamizole, phenylephrine, pseudoephedrine, and codeine IBSpharm CM-1 HPLC column IBS

Figure 1. Group HPLC determination of acetaminophen (paracetamol), caffeine, guaifenesin, phenobarbital, metamizole, phenylephrine, pseudoephedrine, and codeine.

SP: 250x4.6 IBSpharm CM-1

MP: Acetonitrile-(50 mM NH4H2PO4) 20:80

Flow rate: 1.0 ml/min

Column temperature: 25 °C

Example 1

Group HPLC determination of ‘Sudafed’ preparation components. Flow rate: 2.0 ml/min.

HPLC analysis phenylephrine, acetaminophen, guaifenesin IBSpharm CM-1 HPLC column IBS

Example 2

Group HPLC determination of ‘Sedalgin-Neo’ preparation components. Flow rate: 2.0 ml/min.

HPLC analysis acetaminophen, metamizole, codeine, caffeine, phenobarbital IBSpharm CM-1 HPLC column IBS

Inquiry letter   Please, use the form below to send the inquiry letter. We will be glad to receive it, and we undertake to reply no later than 36 hours. Information on the price and stock availability will be attached to our answer. 
You can also send your inquire letter directly to our e-mail sales@hplc.today. Thank you! 

 
  IBSnutri PP-1   

IBSpharm PP-1: Polyphenolic compounds

 

Scope:   The stationary phase is designed for determination of polyphenolic compounds of various classes in plant raw materials, extracts and dietary supplements using isocratic elution mode.

Figure 1. HPLC determination of three main Ginkgo Biloba flavonols (quercetin, kaempferol, isoramnetin) in hydrolyzate of the dietary supplement containing the extract of Ginkgo Biloba leaves.

SP: 250x4.6 IBSnutri PP-1

MP: Acetonitrile-(50 mM NH4H2PO4 + 0.5% H3PO4) 50:50

Flow rate: 1.0 ml/min

Column temperature: 25 °C

Inquiry letter   Please, use the form below to send the inquiry letter. We will be glad to receive it, and we undertake to reply no later than 36 hours. Information on the price and stock availability will be attached to our answer. 
You can also send your inquire letter directly to our e-mail sales@hplc.today. Thank you! 

 
  IBSpharm IN-1   

IBSpharm IN-1: Non-steroidal anti-inflammatory drugs

 

Scope:   The stationary phase is designed for determination of non-steroidal anti-inflammatory drugs: acetylsalicylic acid, ketoprofen, naproxen, ibuprofen and ketorolac using isocratic elution mode.

HPLC analysis of acetylsalicylic acid, ketoprofen, naproxen, ibuprofen, and ketorolac IBSpharm IN-1 HPLC column IBS

Figure 1. Group HPLC determination of acetylsalicylic acid, ketoprofen, naproxen, ibuprofen, and ketorolac.

SP: 250x4.6 IBS.pharm IN-1

MP: Acetonitrile-(50 mM NH4H2PO4 + 0.1% H3PO4) 55:45

Flow rate: 1.0 ml/min

Column temperature: 25 °C

Inquiry letter   Please, use the form below to send the inquiry letter. We will be glad to receive it, and we undertake to reply no later than 36 hours. Information on the price and stock availability will be attached to our answer. 
You can also send your inquire letter directly to our e-mail sales@hplc.today. Thank you! 

 
  IBSpharm HL-1   

IBSpharm HL-1: Lipid-lowering drugs

 

Scope:   The stationary phase is designed for determination of statins (atorvastatin, rosuvastatin) and bezafibrate using isocratic elution mode.

HPLC analysis of atorvastatin, rosuvastatin and bezafibrate IBSpharm HL-1 HPLC column IBS

Figure 1. Group HPLC determination of atorvastatin, rosuvastatin and bezafibrate.

SP: 250x4.6 IBSpharm HL-1

MP: Acetonitrile-(50 mM NH4H2PO4 + 0.1% H3PO4) 50:50

Flow rate: 1.0 ml/min

Column temperature: 25 °C

Inquiry letter   Please, use the form below to send the inquiry letter. We will be glad to receive it, and we undertake to reply no later than 36 hours. Information on the price and stock availability will be attached to our answer. 
You can also send your inquire letter directly to our e-mail sales@hplc.today. Thank you! 

 
  IBSpharm HS-1  

IBSpharm HS-1: Antihistamine drugs

 

Scope:   The stationary phase is designed for highly selective determination of antihistamine drugs using isocratic elution mode.

HPLC analysis of phenyramine, chlorpheniramine and doxylamine in caugh cold flu medications IBSpharm HS-1 HPLC column IBS

Figure 1. Selective HPLC determination of phenyramine, chlorpheniramine and doxylamine in cold and flu medications.

SP: 250x4.6 IBSpharm HS-1

MP: Acetonitrile-(15mM NH4H2PO4) 85:15

Flow rate: 1.0 ml/min

Column temperature: 25 °C

Example 1

HPLC determination of cetirizine and desloratadine.

Example 2 

 

Group HPLC determination of ‘Pentalgin’ preparation components.

HPLC analysis of antihistamine drugs (cetirizine and desloratadine) IBSpharm HS-1 HPLC column IBS
HPLC analysis naproxen, acetaminophen, caffeine, drotaverine, pheniramine IBSpharm HS-1 HPLC column IBS

Inquiry letter   Please, use the form below to send the inquiry letter. We will be glad to receive it, and we undertake to reply no later than 36 hours. Information on the price and stock availability will be attached to our answer. 
You can also send your inquire letter directly to our e-mail sales@hplc.today. Thank you! 

 

IBSpharm CN-1: Medical cannabis compounds

 

Scope:   The stationary phase is designed for the determination of cannabinoids in medical cannabis samples in isocratic elution mode.

HPLC analysis of cannabinoids: cannabidiolic acid (CBDA), d9-tetrahydro-cannabinolic acid (THCA), cannabinol (CBN), canncabichromene (CBC), cannabidiol (CBD), d8-tetrahydrocannabinol (THC8), d9-tetrahydrocannabinol (THC9), cannabigerol (CBG) IBSpharm CN-1 HPLC column IBS

Figure 1. Group HPLC determination of medical cannabis compounds: cannabidiolic acid (CBDA), d9-tetrahydro-cannabinolic acid (THCA), cannabinol (CBN), canncabichromene (CBC), cannabidiol (CBD), d8-tetrahydrocannabinol (THC8), d9-tetrahydrocannabinol (THC9), cannabigerol (CBG).

SP: 250x4.6 IBSpharm CN-1 

MP: Methanol-(100mM NH4OOCH) 85:15

Flow rate: 1.0 ml/min

Column temperature: 25 °C

Benefits

1. The separation developed under isocratic elution conditions is ideal choice for routine analysis of medical cannabis samples. It is much more robust as compared with separations in gradient elution mode.

2. The separation can be scaled up and used for semi-preparative and preparative isolation of different cannabinoids from medical cannabis samples.

3. Excellent separation of all  major cannabinoid compounds, including THC isomers.

4. To apply mass-spectrometric detection, one should decrease ammonium formate concentration to some acceptible level. High salt concentration is required in case of HPLC-UV analysis only, since it provides high columns loadability, which is not necessary in HPLC-MS. 

Inquiry letter   Please, use the form below to send the inquiry letter. We will be glad to receive it, and we undertake to reply no later than 36 hours. Information on the price and stock availability will be attached to our answer. 
You can also send your inquire letter directly to our e-mail sales@hplc.today. Thank you! 

  IBSpharm CN-1  
 

IBSpharm CA-1: Catecholamines and structurally similar compounds

 

Scope:   The stationary phase is designed for the determination of biogenic amines (norepinephrine, epinephrine, DOPA, dopamine) and structurally similar compounds (serotonin, phenylephrine, pseudoephedrine, orciprenaline, fenoterol, salbutamol) in medicinal preparations and biological samples in isocratic elution mode using either UV, or electrochemical or mass-spectrometric detection.

HPLC analysis of catecholamines: norepinephrine, epinephrine, DOPA, dopamine, serotonin IBSpharm CA-1 IBS
HPLC analysis phenylephrine in drug syrup containing acetaminophen (paracetamol) IBSpharm CA-1 IBS

Figure 1. Group HPLC determination of norepinephrine, epinephrine, DOPA, dopamine and serotonin. To apply mass-spectrometric detection, ammonium dihydrogen phosphate should be replaced by ammonium formate while maintaining the same salt concentration.

SP: 250x4.6 IBSpharm CA-1

MP: Acetonitrile (15mM NH4H2PO4) 7:93

Flow rate: 0.8 ml/min

Column temperature: 45 °C

Benefits

1. The mobile phase does not contain EDTA, citrate and other additives harmful for the chromatographic column used and the pump of HPLC system.

2. The mobile phase based on ammonium dihydrogen phosphate is also compatible with electrochemical detection. To apply mass-spectrometric detection, ammonium dihydrogen phosphate should be replaced by ammonium formate while maintaining the same salt concentration.

Figure 2. HPLC determination of phenylephrine in drug syrup containing acetaminophen (paracetamol).

SP: 250x4.6 IBSpharm CA-1

MP: Acetonitrile (15 mM NH4H2PO4) 15:85

Flow rate: 1.5 ml/min

Column temperature: 45 °C

Inquiry letter   Please, use the form below to send the inquiry letter. We will be glad to receive it, and we undertake to reply no later than 36 hours. Information on the price and stock availability will be attached to our answer. 
You can also send your inquire letter directly to our e-mail sales@hplc.today. Thank you! 

  IBSpharm CA-1