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    Ready-to-Use HPLC Applications

Simultaneous Isocratic Determination of Acesulfame, Succharine, Aspartame, Caffeine, Benzoic and Sorbic Acids In Non-Alcoholic Beverages, Beverage Concentrates and Syrups Using HPLC-UV 

Document #031020-09. Revision 01, 10 March 2020



Acesulfame, Succharine, Aspartame, Caffeine, Benzoic acid, Sorbic acid.

Scope of The Method

The method is intended for the isocratic determination of acesulfame, succharine, aspartame, caffeine, benzoic and sorbic acids in non-alcoholic beverages, beverage concentrates and syrups using simple isocratic 400 bar HPLC system with a conventional UV detector.
      The method is capable to determine analytes in the presence of the most of synthetic dyes.

Minimum System Requirements
Solvent Delivery System: Isocratic HPLC pump with 400 bar upper back-pressure limit 
Detector: Single wavelength UV detector
Column Oven: Standard column oven

Compartible with all HPLC systems: Agilent, Shimadzu, Waters, Thermo, etc.

General Information On The Method
Elution Mode: isocratic, Detection: UV, Actual Analysis Time: 15.0 minutes, Back-pressure: typically < 100 bar, HPLC column manufacturer: Thermo Dionex, USA >>


Acesulfame, Succharine, Aspartame, Caffeine, Benzoic acid, Sorbic acid, HPLC, Sweeteners, Agilent, Shimadzu, Waters, Thermo Scientific

Fig. 1. Isocratic determination of five catecholamines and serotonin. Detection: UV 210 nm, 260 nm.
1. Acesulfame,  2. Succharine, 3. Aspartame, 4. Caffeine, 5. Benzoic acid, 6. Sorbic acid.

Acesulfame, Succharine, Aspartame, Caffeine, Benzoic acid, Sorbic acid


The method may require soft adjustment to provide an excellent result for a particular sample. Retention and separation selectivity tuning recommendations are given in the application.

        We can fine-tune method performance to meet your requirements upon request.

        We can develop the complete HPLC method based on this application upon request.

Table of contents

Description of The method  5
Name of The Method 5
Scope of The Method  5
Main Characteritics of The Method 5
Minimum System Requirements 5
Analyte(s) 6
Standard HPLC Conditions 6
Typical Chromatogram(s) 7
Suitable HPLC Column(s) 7
Demonstration of Specificity 9
Quickstart Steps 9
Mobile Phase Preparation 9
Column Washing And Storage 9
Column Conditioning 9
Testing The Column Performance 9
Fine-Tuning Retention And Selectivity


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