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Simultaneous Isocratic Determination of Eight Antihypertensive Drugs Including Alfa & Beta Blockers, CCBs, ARBs And Diuretics Using HPLC-UV
Document #030420-06. Revision 01, 04 March 2020
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Bisoprolol, Hydrochlorothiazide, Doxazosin, Amlodipine, Furosemide, Indapamide, Nifedipine, Valsartan.
Scope of The Method
The method is intended for the isocratic determination of eight antihypertensive drugs including alfa & beta blockers, CCBs, ARBs and diuretics (bisoprolol, hydrochlorothiazide, doxazosin, amlodipine, furosemide, indapamide, nifedipine, valsartan) in various pharmaceutical and biological samples using simple isocratic 400 bar HPLC system with a conventional UV detector.
Minimum System Requirements
Solvent Delivery System: Isocratic HPLC pump with 400 bar upper back-pressure limit
Detector: Single wavelength UV detector
Column Oven: Preferable to ensure retention time stability
Compartible with all HPLC systems: Agilent, Shimadzu, Waters, Thermo, etc.
General Information On The Method
Elution Mode: isocratic, Detection: UV, Actual Analysis Time & Back-pressure: 10 minutes/< 200 bar for 150x4.6 3um; 22 minutes/< 330 bar for 250x4.6 3um, HPLC column manufacturer: Advanced Chromatography Technologies, Scotland >>
Fig. 1. Figure 1. Isocratic determination of eight antihypertensive drugs including alfa & beta blockers, CCBs, ARBs and diuretics.
Detection: UV 225 nm + 235 nm.
1. Bisoprolol, 2. Hydrochlorothiazide, 3. Doxazosin, 4. Amlodipine, 5. Furosemide, 6. Indapamide, 7. Nifedipine, 8. Valsartan.
The method may require soft adjustment to provide an excellent result for a particular sample. Retention and separation selectivity tuning recommendations are given in the application.
We can fine-tune method performance to meet your requirements upon request.
We can develop the complete HPLC method based on this application upon request.
Table of contents
Description of The method 5
Name of The Method 5
Scope of The Method 5
Main Characteritics of The Method 5
Minimum System Requirements 5
Standard HPLC Conditions 6
Typical Chromatogram(s) 7
Suitable HPLC Column(s) 7
Quickstart Steps 8
Mobile Phase Preparation 8
Column Washing And Storage 8
Column Conditioning 8
Testing The Column Performance 8
Fine-Tuning Retention 10