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Simultaneous Isocratic Determination of Eight Antihypertensive Drugs Including Alfa & Beta Blockers, CCBs, ARBs And Diuretics Using HPLC-UV

Document #030420-06. Revision 01, 04 March 2020



Bisoprolol, Hydrochlorothiazide, Doxazosin, Amlodipine, Furosemide, Indapamide, Nifedipine, Valsartan.

Scope of The Method

The method is intended for the isocratic determination of eight antihypertensive drugs including alfa & beta blockers, CCBs, ARBs and diuretics (bisoprolol, hydrochlorothiazide, doxazosin, amlodipine, furosemide, indapamide, nifedipine, valsartan) in various pharmaceutical and biological samples using simple isocratic 400 bar HPLC system with a conventional UV detector.

Minimum System Requirements
Solvent Delivery System: Isocratic HPLC pump with 400 bar upper back-pressure limit 
Detector: Single wavelength UV detector
Column Oven: Preferable to ensure retention time stability

Compartible with all HPLC systems: Agilent, Shimadzu, Waters, Thermo, etc.

General Information On The Method
Elution Mode: isocratic,  Detection: UV, Actual Analysis Time & Back-pressure: 10 minutes/< 200 bar for 150x4.6 3um; 22 minutes/< 330 bar for 250x4.6 3um, HPLC column manufacturer: Advanced Chromatography Technologies, Scotland >>


Fig. 1. Figure 1. Isocratic determination of eight antihypertensive drugs including alfa & beta blockers, CCBs, ARBs and diuretics.
Detection: UV 225 nm + 235 nm.

1. Bisoprolol,  2. Hydrochlorothiazide, 3. Doxazosin, 4. Amlodipine, 5. Furosemide, 6. Indapamide, 7. Nifedipine, 8. Valsartan.


The method may require soft adjustment to provide an excellent result for a particular sample. Retention and separation selectivity tuning recommendations are given in the application.

        We can fine-tune method performance to meet your requirements upon request.

        We can develop the complete HPLC method based on this application upon request.

Table of contents

Description of The method  5
Name of The Method 5
Scope of The Method  5
Main Characteritics of The Method 5
Minimum System Requirements 5
Analyte(s) 6
Standard HPLC Conditions 6
Typical Chromatogram(s) 7
Suitable HPLC Column(s) 7
Quickstart Steps 8
Mobile Phase Preparation 8
Column Washing And Storage 8
Column Conditioning 8
Testing The Column Performance 8
Fine-Tuning Retention  10