top of page
    Ready-to-Use HPLC Applications

Simultaneous specific determination of six antihistamine drugs using isocratic HPLC-UV

Document #022920-02. Revision 01, 29 February 2020



Cetirizine, Chloropyramine, Chlorpheniramine, Pheniramine, Desloratadine, Doxylamine.

Scope of The Method

The method is intended for the highly selective determination of six antihistamine drugs (cetirizine, chloropyramine, chlorpheniramine, pheniramine, desloratadine, doxylamine) in various pharmaceutical and biological samples using simple isocratic 400 bar HPLC system with a conventional UV detector.
       The method is capable to determine antihistamines in complex matrices that contain any neutral or acidic matrix compounds, as well as various basic compounds like drotaverine, dextromethorphan, phenylephrine, etc.
      The method is not capable of determining loratadine.

Minimum System Requirements
Solvent Delivery System: Isocratic HPLC pump with 400 bar upper back-pressure limit 
Detector: Single wavelength UV detector
Column Oven: Preferable to ensure retention time stability

Compartible with all HPLC systems: Agilent, Shimadzu, Waters, Thermo, etc.

General Information On The Method
Elution Mode: isocratic,  Detection: UV, Actual Analysis Time: 6.5 minutes at 1.5 mL/min, Back-pressure: typically < 300 bar at 1.5 mL/min, HPLC column manufacturer: Advanced Chromatography Technologies, Scotland >>


Cetirizine, Chloropyramine, Chlorpheniramine, Pheniramine, Desloratadine, Doxylamine, HPLC, Agilent, Shimadzu, Waters, Thermo Scientific

Fig. 1. Specific determination of six antihistamines. Sample: mixture of six medications; each of them contains one of the analytes.

Detection: UV 260 nm.

Identified matrix components: A. Drotaverine, B. Dextromethorphan.
1. Cetirizine, 2. Chloropyramine, 3. Chlorpheniramine, 4. Pheniramine, 5. Desloratadine, 6. Doxylamine.

Cetirizine, Chloropyramine, Chlorpheniramine, Pheniramine, Desloratadine, Doxylamine


The method may require soft adjustment to provide an excellent result for a particular sample. Retention and separation selectivity tuning recommendations are given in the application.

        We can fine-tune method performance to meet your requirements upon request.

        We can develop the complete HPLC method based on this application upon request.

Table of contents

Description of The method  5
Name of The Method 5
Scope of The Method  5
Main Characteritics of The Method 5
Minimum System Requirements 5
Analyte(s) 6
Standard HPLC Conditions 6
Typical Chromatogram(s) 7
Suitable HPLC Column(s) 7
Demonstration of Specificity 8
Quickstart Steps 9
Mobile Phase Preparation 9
Column Washing And Storage 9
Column Conditioning 9
Testing The Column Performance 9
Fine-Tuning Retention And Selectivity 10


bottom of page